Molecular Weight 646.36 . Ensure that the solutions are mixed well. 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the mic... SDS. Explain why the acid - base indicator bromothymol blue turns yellow when added to dilute HCl but blue when added to dilute NaOH. NACRES NA.47 Answer Save. 1 decade ago. Solutions of different concentration of the indicator were used. Indicator: pH Range: pKa: Acid Form: Base Form: Methy Violet: 0.0 - 1.6: 0.8: Yellow: Blue: Thymol Blue: 1.2 - 2.8: 1.6: Red: Yellow: Methyl Yellow: 29. 2 Answers. Computational methods, such as SQUAD (84) 16 and SPECFIT/32, and some new methods Into each of the cuvettes place 3 mL of the solutions of the various pH values (hydrochloric acid, the buffer solutions with pH values 6.0, 7.0 and 8.0, and sodium hydroxide solution). Using bromothymol blue you wouldn't be able to distinquish between anacid with pH 4 from an acid with pH 5. Favorite Answer. Bromothymol blue, Powder ACS. The indicator (HIn) is a monoprotic acid and we can represent its dissociation as follows: HIn → H+ + In- … (1) The equilibrium expression for such dissociation can be written as. MDL number MFCD00077263. Spectrophotometric determination of the pKa of Bromothymol Blue. Description: Bromothymol Blue (BTB) is a pH indicator (pKa approx. Bromothymol Blue sodium salt powder Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt CAS Number 34722-90-2. EC Number 252-169-7. Molar absorptivity ___ (You will be using this value in your experiment.) They would both appearyellow. Bromothymol blue is an acidic pH chromoionophore where acid to base change corresponds to its neutral to anionic form, respectively. Relevance. Other indicators work in different pH ranges. For this reason it can be electrostatically immobilized on the surface on anion-exchanger resin like Amberlite IRA 401. Place three small drops of bromothymol blue solution into each of the 5 cuvettes. There is a linear relationship between log ( [ln-]/ [Hln]) and pH. Supplier: MP Biomedicals. Assume a cuvet with a 14.45 mm diameter was used. 2. Determination of the pK_a of Bromothymol Blue Use the following graph to determine the molar absorptivity of Bromothymol blue (BTB)? As a pH indicator,bromothymol blue, for example, would be useful between from about pH6.0 to pH 7.6. pH = pKa - log ( [HIn] / [In-] ) … (2) [10] [11] describe multiwavelength analysis and nonlinear least squares regres-sion methods. Acid - Base indicators are either weak acids or weak bases so they exist as molecules in equilibrium with their ions. It shows that pH=pKa where [ln-]= [Hln] or hwere ( [ln-]/ [Hln])=1 or where log ( [ln-]/ [Hln])=0. Meloun et al. ,IC10113005EA,IC10113025EA. Anonymous. Chloroform showed the highest affinity for bromothymol blue, followed by methylene chloride, ethylene dichloride, benzene, toluene, and carbon tetrachloride. Empirical Formula (Hill Notation) C 27 H 27 Br 2 NaO 5 S . 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